Ukora mubijyanye nubushakashatsi bwibinyabuzima?Niba aribyo, noneho ushobora kuba warumvise ibya Sulfo-NHS.Nkuko uruhare rukomeye rwuru ruganda mubushakashatsi rukomeje kumenyekana, uru ruganda rwinjira muri laboratoire nyinshi kwisi.Muri iki kiganiro, turaganira kuri Sulfo-NHS icyo ari cyo n'impamvu ari igikoresho cyagaciro cyane kubiga siyanse y'ibinyabuzima.
Ubwa mbere, Sulfo-NHS ni iki?Izina ni ndende-ndende, reka rero tuyice.Sulfo bisobanura aside sulfonike na NHS bisobanura N-hydroxysuccinimide.Iyo ibyo bice byombi bihujwe,Sulfo-NHSni Byakozwe.Uru ruganda rufite byinshi rukoreshwa mubushakashatsi bwibinyabuzima, ariko kimwe mubintu byingenzi ni ubushobozi bwo guhitamo poroteyine.
Sulfo-NHS ikora mugukora amine yibanze (ni ukuvuga amatsinda -NH2) kumurongo wuruhande rwibisigazwa bya lysine muri proteyine.Mu byingenzi, Sulfo-NHS ihuza poroteyine "tag", ikaborohereza kumenya no gusesengura mubushakashatsi butandukanye.Ibi byavuyemo ibice byinshi byubushakashatsi bushobora gutera imbere hamwe nukuri kandi kurwego rwo hejuru.
None, Sulfo-NHS ikoreshwa iki?Imikoreshereze imwe yuru ruganda ni mubushakashatsi bwikingira.Sulfo-NHS yerekanwe kuranga antibodi na antigene neza, ifungura inzira nshya zo kwiga ibijyanye n’indwara z’umubiri n’indwara.Byongeye kandi,Sulfo-NHSIrashobora gukoreshwa mubushakashatsi bwimikorere ya protein-protein kuko ituma abashakashatsi bamenya vuba kandi byoroshye mugihe poroteyine ebyiri zikorana.
Ahandi hantu Sulfo-NHS ikoreshwa cyane ni proteomics.Proteomics yiga imiterere n'imikorere ya poroteyine zose mu binyabuzima, kandiSulfo-NHSni igikoresho cyingenzi muri iri sesengura.Mugushushanya poroteyine hamwe na Sulfo-NHS, abashakashatsi barashobora gukora ubushakashatsi kugirango babone amakuru arambuye kubyerekeye proteome y’ibinyabuzima runaka, ishobora noneho gufasha kumenya ibimenyetso bishobora kwanduza indwara.
Sulfo-NHS nayo igira uruhare mugutezimbere imiti mishya.Iyo abashakashatsi bagerageza gukora imiti mishya, ni ngombwa kumenya neza ko yibasira poroteyine yagenewe kandi atari izindi poroteyine mu mubiri.UkoreshejeSulfo-NHSguhitamo poroteyine, abashakashatsi barashobora kumenya intego nyayo yibiyobyabwenge bishobora gufasha kwihutisha iterambere ryibiyobyabwenge.
Ngaho rero ufite!Sulfo-NHS ntishobora kuba ijambo rizwi cyane hanze yubumenyi, ariko iyi nteruro irihuta kuba igikoresho cyagaciro mubushakashatsi bwibinyabuzima.Kuva mubushakashatsi bwikingira kugeza kuri proteomics kugeza iterambere ryibiyobyabwenge, Sulfo-NHS ifasha abashakashatsi gutera imbere muri utwo turere kandi twishimiye kubona ibivumbuwe bizaza.
Igihe cyo kohereza: Jun-12-2023